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35%), and reduces tumor progression in OSCC mouse models with minimal toxicity, offering a promising strategy against chemoresistance

GST pull-down assays For GST pull-down assay, a tube of the 1 ml mouse liver cytosol was thawed on ice, centrifuged at 20,000 x g for 20 minutes at 4 C to remove any precipitated material, and the clarified cytosol was then diluted to a total protein concentration of approximately 20 mg/ml with cold homogenization buffer B (homogenization buffer A containing TX-100 at a final concentration of 0.1%)

Springer International Publishing, Cham, pp 195202

This metabolic shift rapidly depletes cellular glycogen reserves, generates excessive lactic acid, and leads to the crushing, unyielding fatigue that characterizes the Long COVID experience